This week I was working with Paul once again on bioinformatics. Paul explained how he sequence the 2 reads in every lane as far as lane 16 over the course of the week while I was at school.
Firstly we had to download all of the information form the servers in order to align the DNA. This was a very slow proses as the file was very large. While waiting for the file to load I looked at some more tutorials online about the history of genetics and DNA. The website that I was using went through all of the individual discoveries by different scientists over the years in relation to genetics. It also showed me some of the Nobel prizes won by scientist working in the field of genetics and what they discovered. A lot of what they discovered we kind of take for granted now but it was very interesting to see the experiments they had to perform in order to come to that conclusion.
Once all of the Results from the DNA sequencing was downloaded we allied all of the files onto one program so that we could compare the lanes and reads equally. some parts of it were very interesting as not all of the lanes matched up. This indicated that there was either a mutation on that read if it may just be a fault. However for some of the mutations we went online to check and clarify. Unfortunately we found no new mutations, so we didn't get a paper published out of that ;)
After our DNA was allied Paul showed me how to create a dot line graph to represent our results.
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| Graph |
Today I learnt that in science you need a lot of evidence in order to come to some sort of conclusion. i.e needing 16 lanes in order to compare the reads. You also cannot take anything for granted, you but check to see if you are correct first. Today has shown me that you must be very precise when researching and carrying out tests.
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